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Composite

Part:BBa_K1315010

Designed by: Dimitrios Michailidis   Group: iGEM14_Dundee   (2014-09-26)

Pseudomonas AutoInducer 1 (PAI-1) Biosensor

This is a composite part that consists of four pre-existing biobricks. It uses the luxR promoter from Pseudomonas aeruginosa in conjunction with LasR to report on the presence of Pseudomonas AutoInducer 1 (PAI-1). The device consists of the tet promoter (BBa_R0040), which is constitutively on and drives production of LasR all the time (BBa_C0179). Only in the presence of PAI-1 does LasR form an active complex and then activate the luxR promoter (BBa_R0062) thus leading to the production of GFP (BBa_E0040).

Dundee PAI LuxR.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 382
    Illegal AgeI site found at 579
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 789
    Illegal BsaI.rc site found at 1496


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